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avidin biotin complex kit reagents  (Vector Laboratories)


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    Vector Laboratories avidin biotin complex kit reagents
    Avidin Biotin Complex Kit Reagents, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 11559 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/avidin+biotin+blocking+reagents/Avidin%2FBiotin+Blocking+Kit/10__1016_slash_j__bramec__2026__202544-102-75-79
    Average 96 stars, based on 11559 article reviews
    avidin biotin complex kit reagents - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Incubation:

    Article Title: Nystagmus Associated With the Absence of MYOD Expression Across the Lifespan in Extraocular and Limb Muscles
    Article Snippet: .. Slides were incubated in blocking serum, followed by incubation in avidin/biotin blocking reagents (Vector; SP-2001). .. The sections were incubated in primary antibody against type 1 MyHC (1:50; myh7 ; Novus NBP2-94079) overnight at 4°C.

    Article Title: Nystagmus Associated With the Absence of MYOD Expression Across the Lifespan in Extraocular and Limb Muscles.
    Article Snippet: .. Slides were incubated in blocking serum, followed by incubation in avidin/biotin blocking reagents (Vector; SP-2001). .. The sections were incubated in primary antibody against type 1 MyHC (1:50; myh7; Novus NBP2-94079) overnight at 4°C.

    Article Title: Adaptability of the Immature Ocular Motor Control System: Unilateral IGF-1 Medial Rectus Treatment
    Article Snippet: For further analysis of myosin expression, every 40th section was stained with antibody to embryonic (1:20 dilution) and neonatal (1:20 dilution) MyHC (Vector Laboratories). .. Sections were blocked for nonspecific binding with goat serum and avidin-biotin blocking reagents (Vector Laboratories), incubated in the primary antibody overnight at 4°C, rinsed, and then incubated with the ABC reagents (Vectastain Elite; Vector Laboratories). ..

    Article Title: Toxicology Study of Intra-Cisterna Magna Adeno-Associated Virus 9 Expressing Iduronate-2-Sulfatase in Rhesus Macaques
    Article Snippet: .. For immunohistochemistry to detect T (CD3) and B (CD20) lymphocytes, paraffin sections were deparaffinized through a series of xylene and ethanol treatments, boiled in a microwave for 6 min in 10 mM citrate buffer (pH 6.0), and treated sequentially with 2% H 2 O 2 (15 min; Sigma-Aldrich, St. Louis, MO), avidin-biotin blocking reagents (15 min each; Vector Laboratories, Burlingame, CA), and blocking buffer (1% donkey serum in PBS + 0.2% Triton for 10 min) followed by incubation with primary (1 hr) and biotinylated secondary antibodies (45 min; donkey antibodies from Jackson ImmunoResearch, West Grove, PA) diluted in blocking buffer. .. Primary antibodies were rabbit polyclonal against CD3 (A045229-2; Agilent Technologies, Santa Clara, CA) and CD20 (PA5-16701; Life Technologies, Carlsbad, CA).

    Blocking Assay:

    Article Title: Nystagmus Associated With the Absence of MYOD Expression Across the Lifespan in Extraocular and Limb Muscles
    Article Snippet: .. Slides were incubated in blocking serum, followed by incubation in avidin/biotin blocking reagents (Vector; SP-2001). .. The sections were incubated in primary antibody against type 1 MyHC (1:50; myh7 ; Novus NBP2-94079) overnight at 4°C.

    Article Title: Myeloid-Specific Tristetraprolin Deficiency in Mice Results in Extreme Lipopolysaccharide Sensitivity in an Otherwise Minimal Phenotype
    Article Snippet: For immunostaining, sections were incubated in 3% H 2 O 2 to inactivate endogenous peroxidases, followed by antigen retrieval with heat and pressure in citrate butter (Biocare Medical, CA). .. Endogenous biotin was blocked with Avidin-Biotin blocking reagents (Vector Laboratories, Burlingame, CA). .. Sections were then incubated with anti-CD45 antibody (GeneTex, San Antonio, TX) for 60 min at a 1:250 dilution, followed by peroxidase-conjugated streptavidin SS labeling (Biogenex Laboratories, San Ramon, CA) for 30 min. Immunolabeled antigen-antibody complexes were visualized using diaminobenzidine.

    Article Title: Nystagmus Associated With the Absence of MYOD Expression Across the Lifespan in Extraocular and Limb Muscles.
    Article Snippet: .. Slides were incubated in blocking serum, followed by incubation in avidin/biotin blocking reagents (Vector; SP-2001). .. The sections were incubated in primary antibody against type 1 MyHC (1:50; myh7; Novus NBP2-94079) overnight at 4°C.

    Article Title: Adaptability of the Immature Ocular Motor Control System: Unilateral IGF-1 Medial Rectus Treatment
    Article Snippet: For further analysis of myosin expression, every 40th section was stained with antibody to embryonic (1:20 dilution) and neonatal (1:20 dilution) MyHC (Vector Laboratories). .. Sections were blocked for nonspecific binding with goat serum and avidin-biotin blocking reagents (Vector Laboratories), incubated in the primary antibody overnight at 4°C, rinsed, and then incubated with the ABC reagents (Vectastain Elite; Vector Laboratories). ..

    Article Title: Stratification of ovarian tumor pathology by expression of programmed cell death-1 (PD-1) and PD-ligand- 1 (PD-L1) in ovarian cancer
    Article Snippet: Sections were washed in 0.1% tween in Dulbecco’s phosphate buffered saline (DPBS; 1X, Lonza, Walkersville, MD) and then blocked in 0.4% hydrogen peroxide in DPBS, followed by blocking in 10% goat serum (S1000, Vector Laboratories, Burlingame, CA) for 1 h. Antigen retrieval for FoxP3, CD3 and CD8 was performed by boiling sections in a pressure cooker for 5 min in Reveal Decloaker (RV1000G1, Biocare Medical, Concord, CA). .. After washing in DPBS, sections were blocked in 0.4% hydrogen peroxide in DPBS for 20 min, 10% goat serum or 10% horse serum (S1000 or S2000 respectively, Vector Laboratories) for 20 min, and then in Avidin/ Biotin blocking reagents (SP 2001, Vector Laboratories) to further reduce non-specific staining of primary antibody (FoxP3, CD3, or CD8). ..

    Article Title: Tumor-infiltrating myeloid cells induce tumor cell resistance to cytotoxic T cells in mice
    Article Snippet: .. For double staining, the sections were blocked by Avidin-Biotin blocking reagents (Vector Laboratories) before the second staining process. ..

    Article Title: VEGF Secretion Drives Bone Formation in Classical MAP2K1+ Melorheostosis.
    Article Snippet: Patients with classical melorheostosis exhibit exuberant bone overgrowth in the appendicular skeleton, resulting in pain and deformity with no known treatment.. Most patients have somatic, mosaic mutations inMAP2K1 (encoding the MEK1 protein) in osteoblasts and overlying skin.. As with most rare bone diseases, lack of affected tissue has limited the opportunity to understand how the mutation results in excess bone formation.

    Article Title: Toxicology Study of Intra-Cisterna Magna Adeno-Associated Virus 9 Expressing Iduronate-2-Sulfatase in Rhesus Macaques
    Article Snippet: .. For immunohistochemistry to detect T (CD3) and B (CD20) lymphocytes, paraffin sections were deparaffinized through a series of xylene and ethanol treatments, boiled in a microwave for 6 min in 10 mM citrate buffer (pH 6.0), and treated sequentially with 2% H 2 O 2 (15 min; Sigma-Aldrich, St. Louis, MO), avidin-biotin blocking reagents (15 min each; Vector Laboratories, Burlingame, CA), and blocking buffer (1% donkey serum in PBS + 0.2% Triton for 10 min) followed by incubation with primary (1 hr) and biotinylated secondary antibodies (45 min; donkey antibodies from Jackson ImmunoResearch, West Grove, PA) diluted in blocking buffer. .. Primary antibodies were rabbit polyclonal against CD3 (A045229-2; Agilent Technologies, Santa Clara, CA) and CD20 (PA5-16701; Life Technologies, Carlsbad, CA).

    Avidin-Biotin Assay:

    Article Title: Nystagmus Associated With the Absence of MYOD Expression Across the Lifespan in Extraocular and Limb Muscles
    Article Snippet: .. Slides were incubated in blocking serum, followed by incubation in avidin/biotin blocking reagents (Vector; SP-2001). .. The sections were incubated in primary antibody against type 1 MyHC (1:50; myh7 ; Novus NBP2-94079) overnight at 4°C.

    Article Title: Myeloid-Specific Tristetraprolin Deficiency in Mice Results in Extreme Lipopolysaccharide Sensitivity in an Otherwise Minimal Phenotype
    Article Snippet: For immunostaining, sections were incubated in 3% H 2 O 2 to inactivate endogenous peroxidases, followed by antigen retrieval with heat and pressure in citrate butter (Biocare Medical, CA). .. Endogenous biotin was blocked with Avidin-Biotin blocking reagents (Vector Laboratories, Burlingame, CA). .. Sections were then incubated with anti-CD45 antibody (GeneTex, San Antonio, TX) for 60 min at a 1:250 dilution, followed by peroxidase-conjugated streptavidin SS labeling (Biogenex Laboratories, San Ramon, CA) for 30 min. Immunolabeled antigen-antibody complexes were visualized using diaminobenzidine.

    Article Title: Nystagmus Associated With the Absence of MYOD Expression Across the Lifespan in Extraocular and Limb Muscles.
    Article Snippet: .. Slides were incubated in blocking serum, followed by incubation in avidin/biotin blocking reagents (Vector; SP-2001). .. The sections were incubated in primary antibody against type 1 MyHC (1:50; myh7; Novus NBP2-94079) overnight at 4°C.

    Article Title: Adaptability of the Immature Ocular Motor Control System: Unilateral IGF-1 Medial Rectus Treatment
    Article Snippet: For further analysis of myosin expression, every 40th section was stained with antibody to embryonic (1:20 dilution) and neonatal (1:20 dilution) MyHC (Vector Laboratories). .. Sections were blocked for nonspecific binding with goat serum and avidin-biotin blocking reagents (Vector Laboratories), incubated in the primary antibody overnight at 4°C, rinsed, and then incubated with the ABC reagents (Vectastain Elite; Vector Laboratories). ..

    Article Title: Stratification of ovarian tumor pathology by expression of programmed cell death-1 (PD-1) and PD-ligand- 1 (PD-L1) in ovarian cancer
    Article Snippet: Sections were washed in 0.1% tween in Dulbecco’s phosphate buffered saline (DPBS; 1X, Lonza, Walkersville, MD) and then blocked in 0.4% hydrogen peroxide in DPBS, followed by blocking in 10% goat serum (S1000, Vector Laboratories, Burlingame, CA) for 1 h. Antigen retrieval for FoxP3, CD3 and CD8 was performed by boiling sections in a pressure cooker for 5 min in Reveal Decloaker (RV1000G1, Biocare Medical, Concord, CA). .. After washing in DPBS, sections were blocked in 0.4% hydrogen peroxide in DPBS for 20 min, 10% goat serum or 10% horse serum (S1000 or S2000 respectively, Vector Laboratories) for 20 min, and then in Avidin/ Biotin blocking reagents (SP 2001, Vector Laboratories) to further reduce non-specific staining of primary antibody (FoxP3, CD3, or CD8). ..

    Article Title: Tumor-infiltrating myeloid cells induce tumor cell resistance to cytotoxic T cells in mice
    Article Snippet: .. For double staining, the sections were blocked by Avidin-Biotin blocking reagents (Vector Laboratories) before the second staining process. ..

    Article Title: VEGF Secretion Drives Bone Formation in Classical MAP2K1+ Melorheostosis.
    Article Snippet: Patients with classical melorheostosis exhibit exuberant bone overgrowth in the appendicular skeleton, resulting in pain and deformity with no known treatment.. Most patients have somatic, mosaic mutations inMAP2K1 (encoding the MEK1 protein) in osteoblasts and overlying skin.. As with most rare bone diseases, lack of affected tissue has limited the opportunity to understand how the mutation results in excess bone formation.

    Article Title: Toxicology Study of Intra-Cisterna Magna Adeno-Associated Virus 9 Expressing Iduronate-2-Sulfatase in Rhesus Macaques
    Article Snippet: .. For immunohistochemistry to detect T (CD3) and B (CD20) lymphocytes, paraffin sections were deparaffinized through a series of xylene and ethanol treatments, boiled in a microwave for 6 min in 10 mM citrate buffer (pH 6.0), and treated sequentially with 2% H 2 O 2 (15 min; Sigma-Aldrich, St. Louis, MO), avidin-biotin blocking reagents (15 min each; Vector Laboratories, Burlingame, CA), and blocking buffer (1% donkey serum in PBS + 0.2% Triton for 10 min) followed by incubation with primary (1 hr) and biotinylated secondary antibodies (45 min; donkey antibodies from Jackson ImmunoResearch, West Grove, PA) diluted in blocking buffer. .. Primary antibodies were rabbit polyclonal against CD3 (A045229-2; Agilent Technologies, Santa Clara, CA) and CD20 (PA5-16701; Life Technologies, Carlsbad, CA).

    Binding Assay:

    Article Title: Adaptability of the Immature Ocular Motor Control System: Unilateral IGF-1 Medial Rectus Treatment
    Article Snippet: For further analysis of myosin expression, every 40th section was stained with antibody to embryonic (1:20 dilution) and neonatal (1:20 dilution) MyHC (Vector Laboratories). .. Sections were blocked for nonspecific binding with goat serum and avidin-biotin blocking reagents (Vector Laboratories), incubated in the primary antibody overnight at 4°C, rinsed, and then incubated with the ABC reagents (Vectastain Elite; Vector Laboratories). ..

    Staining:

    Article Title: Stratification of ovarian tumor pathology by expression of programmed cell death-1 (PD-1) and PD-ligand- 1 (PD-L1) in ovarian cancer
    Article Snippet: Sections were washed in 0.1% tween in Dulbecco’s phosphate buffered saline (DPBS; 1X, Lonza, Walkersville, MD) and then blocked in 0.4% hydrogen peroxide in DPBS, followed by blocking in 10% goat serum (S1000, Vector Laboratories, Burlingame, CA) for 1 h. Antigen retrieval for FoxP3, CD3 and CD8 was performed by boiling sections in a pressure cooker for 5 min in Reveal Decloaker (RV1000G1, Biocare Medical, Concord, CA). .. After washing in DPBS, sections were blocked in 0.4% hydrogen peroxide in DPBS for 20 min, 10% goat serum or 10% horse serum (S1000 or S2000 respectively, Vector Laboratories) for 20 min, and then in Avidin/ Biotin blocking reagents (SP 2001, Vector Laboratories) to further reduce non-specific staining of primary antibody (FoxP3, CD3, or CD8). ..

    Article Title: Tumor-infiltrating myeloid cells induce tumor cell resistance to cytotoxic T cells in mice
    Article Snippet: .. For double staining, the sections were blocked by Avidin-Biotin blocking reagents (Vector Laboratories) before the second staining process. ..

    Double Staining:

    Article Title: Tumor-infiltrating myeloid cells induce tumor cell resistance to cytotoxic T cells in mice
    Article Snippet: .. For double staining, the sections were blocked by Avidin-Biotin blocking reagents (Vector Laboratories) before the second staining process. ..

    Immunohistochemistry:

    Article Title: Toxicology Study of Intra-Cisterna Magna Adeno-Associated Virus 9 Expressing Iduronate-2-Sulfatase in Rhesus Macaques
    Article Snippet: .. For immunohistochemistry to detect T (CD3) and B (CD20) lymphocytes, paraffin sections were deparaffinized through a series of xylene and ethanol treatments, boiled in a microwave for 6 min in 10 mM citrate buffer (pH 6.0), and treated sequentially with 2% H 2 O 2 (15 min; Sigma-Aldrich, St. Louis, MO), avidin-biotin blocking reagents (15 min each; Vector Laboratories, Burlingame, CA), and blocking buffer (1% donkey serum in PBS + 0.2% Triton for 10 min) followed by incubation with primary (1 hr) and biotinylated secondary antibodies (45 min; donkey antibodies from Jackson ImmunoResearch, West Grove, PA) diluted in blocking buffer. .. Primary antibodies were rabbit polyclonal against CD3 (A045229-2; Agilent Technologies, Santa Clara, CA) and CD20 (PA5-16701; Life Technologies, Carlsbad, CA).



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